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Over the last decade, the use of unmanned aerial vehicles (UAVs) for plant phenotyping and field crop monitoring has significantly evolved and expanded. These technologies have been particularly valuable for monitoring crop growth and health and for managing abiotic and biotic stresses such as drought, fertilization deficiencies, disease, and bioaggressors. This paper provides a comprehensive review of the progress in UAV‐based plant phenotyping, with a focus on the current use and application of drone technology to gain information on plant growth, development, adaptation, and yield. We reviewed over 200 research articles and discuss the best tools and methodologies for different research purposes, the challenges that need to be overcome, and the major research gaps that remain. First, the review offers a critical focus on elucidating the distinct characteristics of UAV platforms, highlighting the diverse sensor technologies employed and shedding light on the nuances of UAV data acquisition and processing methodologies. Second, it presents a comprehensive analysis of the multiple applications of UAVs in field phenotyping, underscoring the transformative potential of integrating machine learning techniques for plant analysis. Third, it delves into the realm of machine learning applications for plant phenotyping, emphasizing its role in enhancing data analysis and interpretation. Furthermore, the paper extensively examines the open issues and research challenges within the domain, addressing the complexities and limitations faced during data acquisition, processing, and interpretation. Finally, it outlines the future trends and emerging technologies in the field of UAV‐based plant phenotyping, paving the way for innovative advancements and methodologies.more » « lessFree, publicly-accessible full text available December 1, 2025
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Assessing soil organic carbon (SOC) stocks is crucial for understanding the carbon sequestration potential of agroecosystems and for mitigating climate change. This study presents a novel method for assessing SOC and mineral content at various soil depths in sorghum crops using hyperspectral remote sensing. Conducted at Planthaven Farms, MO, the research encompassed ten genotypes across 30 plots, yielding 180 soil samples from six depth intervals (0–150 cm) of bare soil. Chemical analyses determined the SOC and mineral levels, which were then compared to spectral data from HySpex indoor sensors. We utilized time-frequency analysis methods, including discrete wavelet transformation (DWT), continuous wavelet transformation (CWT), and frame transformation along with traditional spectral transformations, specifically fractional derivatives and continuum removal. The analysis revealed the shortwave infrared (SWIR) region, particularly the 1800–2000 nm range, as having the strongest correlations with SOC content (with R2 exceeding 0.8). The visible near-infrared (VNIR) region also provided valuable insights. Models incorporating CWT achieved high accuracy (test R2 exceeding 0.9), while frame transformation achieved strong accuracy (test R2 between 0.7 and 0.8) with fewer features. The random forest regressor (RFR) proved to be most robust, demonstrating superior accuracy and reduced overfitting compared to support vector regression (SVR), partial least squares regression (PLSR), and deep neural network (DNN) models. The models demonstrated the efficacy of hyperspectral data for SOC estimation, suggesting potential for future applications that integrate this data with above-ground biomass to improve SOC mapping across larger scales. This research offers a promising spectral transformation approach for effective carbon management and sustainable agriculture in a changing climate.more » « less
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Ames, Daniel P (Ed.)Hydrological streamline delineation is critical for effective environmental management, influencing agriculture sustainability, river dynamics, watershed planning, and more. This study develops a novel approach to combining transfer learning with convolutional neural networks that capitalize on image-based pre-trained models to improve the accuracy and transferability of streamline delineation. We evaluate the performance of eleven image-based pre-trained models and a baseline model using datasets from Rowan County, North Carolina, and Covington River, Virginia in the USA. Our results demonstrate that when models are adapted to a new area, the fine-tuned ImageNet pre-trained model exhibits superior predictive accuracy, markedly higher than the models trained from scratch or those only fine-tuned on the same area. Moreover, the pre-trained model achieves better smoothness and connectivity between classified streamline channels. These findings underline the effectiveness of transfer learning in enhancing the delineation of hydrological streamlines across varied geographies, offering a scalable solution for accurate and efficient environmental modelling.more » « less
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The potential of artificial intelligence (AI) and machine learning (ML) in agriculture for improving crop yields and reducing the use of water, fertilizers, and pesticides remains a challenge. The goal of this work was to introduce Hyperfidelis, a geospatial software package that provides a comprehensive workflow that includes imagery visualization, feature extraction, zonal statistics, and modeling of key agricultural traits including chlorophyll content, yield, and leaf area index in a ML framework that can be used to improve food security. The platform combines a user-friendly graphical user interface with cutting-edge machine learning techniques, bridging the gap between plant science, agronomy, remote sensing, and data science without requiring users to possess any coding knowledge. Hyperfidelis offers several data engineering and machine learning algorithms that can be employed without scripting, which will prove essential in the plant science community.more » « less
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The food production system is vulnerable to diseases more than ever, and the threat is increasing in an era of climate change that creates more favorable conditions for emerging diseases. Fortunately, scientists and engineers are making great strides to introduce farming innovations to tackle the challenge. Unmanned aerial vehicle (UAV) remote sensing is among the innovations and thus is widely applied for crop health monitoring and phenotyping. This study demonstrated the versatility of aerial remote sensing in diagnosing yellow rust infection in spring wheats in a timely manner and determining an intervenable period to prevent yield loss. A small UAV equipped with an aerial multispectral sensor periodically flew over, and collected remotely sensed images of, an experimental field in Chacabuco (−34.64; −60.46), Argentina during the 2021 growing season. Post-collection images at the plot level were engaged in a thorough feature-engineering process by handcrafting disease-centric vegetation indices (VIs) from the spectral dimension, and grey-level co-occurrence matrix (GLCM) texture features from the spatial dimension. A machine learning pipeline entailing a support vector machine (SVM), random forest (RF), and multilayer perceptron (MLP) was constructed to identify locations of healthy, mild infection, and severe infection plots in the field. A custom 3-dimensional convolutional neural network (3D-CNN) relying on the feature learning mechanism was an alternative prediction method. The study found red-edge (690–740 nm) and near infrared (NIR) (740–1000 nm) as vital spectral bands for distinguishing healthy and severely infected wheats. The carotenoid reflectance index 2 (CRI2), soil-adjusted vegetation index 2 (SAVI2), and GLCM contrast texture at an optimal distance d = 5 and angular direction θ = 135° were the most correlated features. The 3D-CNN-based wheat disease monitoring performed at 60% detection accuracy as early as 40 days after sowing (DAS), when crops were tillering, increasing to 71% and 77% at the later booting and flowering stages (100–120 DAS), and reaching a peak accuracy of 79% for the spectral-spatio-temporal fused data model. The success of early disease diagnosis from low-cost multispectral UAVs not only shed new light on crop breeding and pathology but also aided crop growers by informing them of a prevention period that could potentially preserve 3–7% of the yield at the confidence level of 95%.more » « less
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